PFGE of human DNA: 5-azacytidine improves restriction
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چکیده
منابع مشابه
PFGE of human DNA: 5-azacytidine improves restriction.
Pulsed f i e l d gel electrophoresis (PFGE) (1) of human DNA and the developnent of long-range res tr ic t ion maps are frequently hampered by the cytosine methylation that occurs at OG dinucleot ides in human DNA. This methylation can interfere with re s t r i c t ion and change the s i z e , number and concentration of fragments detected in Southern b l o t s . We have found that these e f f ...
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One of the most readily quantitated indices of myeloid maturation in HL-60 cells is their ability to respond to the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate with increased respiratory burst activity. HL-60 cells exposed to the antileukemic drug, 5-azacytidine (3 to 5 microM) for 24 hr and subsequently cultured in its absence for 2 to 3 days develop an enhanced ability to respond to ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.7.3183